FINE SEPARATION OF PROTEIN ISOFORMS WITH ALTERNATIVE DEVELOPMENT IN 2-DIMENSIONAL ELECTROPHORESIS

Earlier, we had developed a new two-dimensional electrophoresis proced ure, which could show high resolution for tubulin microheterogeneity. The present study was conducted to characterize this electrophoretic m ethod by comparing its resolution with the common two-dimensional elec trophoresis of O'Farrell. The results have shown that our method is su perior to that of O'Farrell's in the separation of proteins with close isoelectric points. Moreover, a further application was tested by usi ng native-gel electrophoresis in the first dimension and isoelectric f ocusing (IEF) in the second dimension. This method provided useful inf ormation on protein components involved in intact protein. (C) 1998 El sevier Science B.V.