I. Wengatz et al., DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTIONOF THE PYRETHROID INSECTICIDE FENPROPATHRIN, Journal of agricultural and food chemistry, 46(6), 1998, pp. 2211-2221
A competitive enzyme-linked immunosorbent assay (ELISA) was developed
for the quantitative detection of fenpropathrin enzyl-2,2,3,3-tetramet
hylcyclopropanecarboxylate]. Polyclonal antisera were isolated from ra
bbits immunized with two different fenpropathrin hapten conjugates. On
e hapten contained an amino function; the other contained a carboxyl g
roup for conjugation to carrier proteins. Mollusk hemocyanins, thyrogl
obulin, and fetuin were used as carrier proteins. The antisera varied
greatly in their affinities for fenpropathrin. A homologous assay syst
em using the coating antigen format was the most sensitive. The IC50 f
or fenpropathrin was 20 mu g/L, and the lower detection limit was 2.5
mu g/L. Pyrethroids, such as phenothrin, permethrin, resmethrin, fenva
lerate, deltamethrin, cyfluthrin, and cypermethrin, and the pyrethroid
metabolites, 3-phenoxybenzoic acid and fenpropathrin acid, did not cr
oss-react significantly in this assay. Ten percent acetone or methanol
and a pH of 4 were determined to be optimum assay conditions. Various
cationic, anionic, and nonionic detergents had no significant effect
on the assay.