DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTIONOF THE PYRETHROID INSECTICIDE FENPROPATHRIN

Citation
I. Wengatz et al., DEVELOPMENT OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTIONOF THE PYRETHROID INSECTICIDE FENPROPATHRIN, Journal of agricultural and food chemistry, 46(6), 1998, pp. 2211-2221
Citations number
36
Categorie Soggetti
Food Science & Tenology",Agriculture,"Chemistry Applied
ISSN journal
00218561
Volume
46
Issue
6
Year of publication
1998
Pages
2211 - 2221
Database
ISI
SICI code
0021-8561(1998)46:6<2211:DOAEFT>2.0.ZU;2-J
Abstract
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative detection of fenpropathrin enzyl-2,2,3,3-tetramet hylcyclopropanecarboxylate]. Polyclonal antisera were isolated from ra bbits immunized with two different fenpropathrin hapten conjugates. On e hapten contained an amino function; the other contained a carboxyl g roup for conjugation to carrier proteins. Mollusk hemocyanins, thyrogl obulin, and fetuin were used as carrier proteins. The antisera varied greatly in their affinities for fenpropathrin. A homologous assay syst em using the coating antigen format was the most sensitive. The IC50 f or fenpropathrin was 20 mu g/L, and the lower detection limit was 2.5 mu g/L. Pyrethroids, such as phenothrin, permethrin, resmethrin, fenva lerate, deltamethrin, cyfluthrin, and cypermethrin, and the pyrethroid metabolites, 3-phenoxybenzoic acid and fenpropathrin acid, did not cr oss-react significantly in this assay. Ten percent acetone or methanol and a pH of 4 were determined to be optimum assay conditions. Various cationic, anionic, and nonionic detergents had no significant effect on the assay.