NEW ENZYMATIC-SYNTHESIS OF 6(3)-MODIFIED MALTOOLIGOSACCHARIDES AND THEIR INHIBITORY ACTIVITIES FOR HUMAN ALPHA-AMYLASES

Authors
UCHIDA R NASU A TOKUTAKE S KASAI K TOBE K YAMAJI N
Citation
R. Uchida et al., NEW ENZYMATIC-SYNTHESIS OF 6(3)-MODIFIED MALTOOLIGOSACCHARIDES AND THEIR INHIBITORY ACTIVITIES FOR HUMAN ALPHA-AMYLASES, Carbohydrate research, 307(1-2), 1998, pp. 69-76
Citations number
24
Categorie Soggetti
Chemistry Applied","Chemistry Inorganic & Nuclear",Biology
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
307
Issue
1-2
Year of publication
1998
Pages
69 - 76
Database
ISI
SICI code
0008-6215(1998)307:1-2<69:NEO6MA>2.0.ZU;2-K
Abstract
Ten new 6(3)-modified maltopentaoses and tetraoses were synthesized by enzymatic reactions utilizing cyclodextrin glycosyltransferase (EC 2. 4.1.19) and subsequent human salivary alpha-amylase (HSA) (EC 3.2.1.1) . Among these compounds, D-glucopyranosyl-(1-->4)-alpha-D-glucopyranos yl-(1 >4)-alpha-D-glucopyranosyl-(1-->4)-D-glucopyranose (11) and >4)- alpha-D-glucopyranosyl-(1-->4)-D-glucopyranose (12) showed strong inhi bitory activities for human pancreatic alpha-amylase (HPA) and HSA. Th e IC50 Of 6(3)-deoxymaltopentaose 11 (8.0x10(-5)M for HPA, 1.0x10(-4)M for HSA) and 6(3)-deoxymaltotetraose 12 (2.0x10(-3)M for HPA, 2.0x10( -3)M for HSA) were lower than that of 6(3)-deoxymaltotriose >4)-alpha- D-glucopyranosyl-(1-->4)-D-glucopyranose 13; 2.0x10-3M for HPA, 4.2x10 (-2)M for HSA]. (C) 1998 Elsevier Science Ltd. All rights reserved.