VIRUS INACTIVATION IN SUPEROXIDE-DISMUTASE PREPARATIONS BY ULTRAVIOLET-LIGHT IRRADIATION

Viral inactivation in superoxide dismutase (SOD) derived from human re d cells was carried out by ultraviolet light C (UVC) irradiation. With 400 J/m(2) UVC irradiation, the titer of canine parvovirus (CPV, a no nenveloped virus), M13 bacteriophage (M13, a nonenveloped phage) and v esicular stomatitis virus (VSV, an enveloped virus), which were spiked into SOD solution, were reduced by >4.6 log(10) (detection limit), 7. 0 log(10) and 6.2 log(10),( )respectively The SOD activity was maintai ned and the band pattern of SOD on an electrophoresis gel was not chan ged even by 1000 J/m(2) UVC irradiation. These results indicate that U VC irradiation is a promising method for the inactivation of both enve loped and nonenveloped viruses in SOD preparations while maintaining i ts activity.