J. Marquez et al., THE ROLE OF THE INTINE AND CYTOPLASM IN THE ACTIVATION AND GERMINATION PROCESSES OF POACEAE POLLEN GRAINS, Grana, 36(6), 1997, pp. 328-342
Ultrastructural modifications of the intine and cytoplasm, during the
maturation, activation and germination processes are described for sev
eral Poaceae pollen grains. Allergenic and antigenic proteins were fou
nd in the non apertural inline during the limes of activation and germ
ination, using TEM immunolabelling. This fact may be related to the fu
nction of the non apertural inline during the processes of pollen acti
vation and pollen tube formation prior to fecundation. Changes in the
granular particles of the cytoplasm are described and their role in po
llen wall development is suggested. The pectic-cellulosic and callosic
lavers of the pollen tube were formed on the degraded intine, and a r
elationship between pollen tube wall development and the substances ex
pelled from the fibrillar particles was observed. The immunolabelling
of the starch granules may be in agreement with their role in the alle
rgenic process.