C. Aguado et al., REAGGREGATION AND BINDING OF CELL-WALL PROTEINS FROM CANDIDA-ALBICANSTO STRUCTURAL POLYSACCHARIDES, Research in microbiology, 149(5), 1998, pp. 327-338
Urea or hot sodium dodecyl sulphate extracted a significant amount of
the same proteins from the matrix of the cell wall of the yeast form a
nd mycelial cells of Candida albicans. Gel filtration analysis of the
urea-extracted proteins revealed that they occurred in the form of lar
ge complexes which were unaffected by up to 8 M urea. Among them, prot
eins en route to becoming covalently associated within the wall scaffo
ld were identified by their reaction with specific antibodies. When ur
ea was removed by dialysis, some of these proteins specifically reasso
ciated into large aggregates which bound strongly with ConA, whereas o
thers remained soluble in smaller associated products. The ability of
some of these proteins to bind to the insoluble wall polysaccharides w
as also assessed. No self-assembling proteins were able to bind to glu
cans and/or chitin. Specificity of the binding to polysaccharides made
of beta-bound glucosyl or N-acetylglucosaminyl residues was determine
d by the competitive effect of several disaccharides, Whereas laminari
biose and diacetylchitobiose were strong inhibitors of protein binding
to both glucan and chitin, lactose, maltose and sucrose were ineffect
ive.