PULMONARY REMOVAL AND PRODUCTION OF ENDOTHELIN IN THE ANESTHETIZED DOG

Citation
J. Dupuis et al., PULMONARY REMOVAL AND PRODUCTION OF ENDOTHELIN IN THE ANESTHETIZED DOG, Journal of applied physiology, 76(2), 1994, pp. 694-700
Citations number
31
Categorie Soggetti
Physiology
ISSN journal
87507587
Volume
76
Issue
2
Year of publication
1994
Pages
694 - 700
Database
ISI
SICI code
8750-7587(1994)76:2<694:PRAPOE>2.0.ZU;2-9
Abstract
The single-bolus multiple-indicator-dilution technique was used to eva luate pulmonary removal of tracer I-125-labeled endothelin-1 in seven anesthetized dogs. Simultaneously, pulmonary arterial and aortic blood samples were obtained and assayed to determine the levels of immunore active endothelin-1. When I-125-endothelin-1 was compared with a plasm a vascular reference (Evans blue dye), there was a single passage mean extraction of 31 +/- 8%. In contrast, there was no significant differ ence between immunoreactive endothelin-1 levels measured in blood samp les from the pulmonary artery and the aorta (1.26 +/- 0.58 and 1.37 +/ - 0.50 pg/ml, respectively; P = 0.47). The absence of an arteriovenous difference for bulk endothelin-1 across the lungs in the presence of tracer data indicating a substantial uptake implies that an amount of endothelin-1 quantitatively more or less equal to that removed is prod uced by the lung. The shapes of the dilution curves suggest that the t racer endothelin uptake by the lung is a one-way process without vascu lar reentry of tracer. We conclude that the dog lung is an important s ite for both uptake and release of endothelin-1.