H. Sager et al., DIFFERENCES BETWEEN B-CELL AND MACROPHAGE TRANSFORMATION BY THE BOVINE PARASITE, THEILERIA-ANNULATA - A CLONAL APPROACH, The Journal of immunology (1950), 161(1), 1998, pp. 335-341
Theileria annulata, a tick-transmitted protozoan parasite, infects and
transforms cells of the hemopoietic system, particularly those of the
B cell and monocyte/macrophage lineages. Here, the effect of infectio
n/transformation on the resulting phenotype was studied using a clonal
approach. Three phenotypes of transformed cell lines could be discern
ed. The first is characterized by surface expression of IgM, CD21, and
the B cell epitopes, B-B2 and B-B8, Ig heavy chain gene rearrangement
, and mRNA expression. Such lines were obtained from fresh and culture
d PBMC and at increased frequency from purified B cells, but never fro
m fetal bone marrow cells. The second phenotype can be distinguished f
rom the first by the absence of Ig heavy chain expression and reduced
surface expression of B cell markers (CD21, B-B2, B-B8), Clones with t
his phenotype were obtained from transformed fetal bone marrow cells o
nly, The third phenotype showed an absence of all of the above B cell
markers, including surface IgM, and a lack of Ig heavy chain gene rear
rangement. The latter clones could be maintained for several weeks aft
er elimination of T. annulata by BW720c treatment, and they reacquired
a macrophage-like phenotype, This implies that parasite-induced dedif
ferentiation is restricted to monocyte/macrophage, and that B cell mar
kers are indicative of cell lineage progeny. Demonstration of surface
IgM on PBMC-derived B cell clones suggests that infection of B cells w
ith T. annulata may be an epigenetic method to immortalize ruminant B
cells of a defined Ag specificity.