EFFECT OF CAMP-RELATED AND CGMP-RELATED DRUGS ON PORCINE CULTURED CILIARY MUSCLE

We studied in this work the compounds which affect the contraction of ciliary muscle and are potential ocular hypotensive drugs, and second messengers produced by the compounds. Using second-passaged porcine cu ltured ciliary muscle, single cell contraction was used for assay. Cel l contraction by carbachol was affected by isobutyl methylxanthine, fo rskolin, dibutyryl cGMP and sodium nitroprusside (SNP). Forskolin incr eased intracellular cAMP while SNP stimulated cGMP formation. The resu lts indicate that these cAMP- or cGMP-related compounds could affect t he contraction of ciliary muscle like prostaglandins and could therefo re have a potential to reduce intraocular pressure.