Al. Lambert et al., TRANSFER OF ALLERGIC AIRWAY RESPONSES WITH SERUM AND LYMPHOCYTES FROMRATS SENSITIZED TO DUST-MITE, American journal of respiratory and critical care medicine, 157(6), 1998, pp. 1991-1999
Citations number
35
Categorie Soggetti
Emergency Medicine & Critical Care","Respiratory System
House dust mite (HDM) antigen is one of the most common allergens asso
ciated with extrinsic asthma, In a model of allergic lung disease, Bro
wn Norway (BN) rats sensitized to HDM with alum and Bordetella pertuss
is adjuvants produce high levels of IgE antibody and experience bronch
oconstriction, increased airway hyperresponsiveness (AHR) to acetylcho
line (ACh), and pulmonary inflammation after antigen challenge. The pu
rpose of this study was to determine whether these asthmatic symptoms
could be transferred from sensitized animals to naive recipients via h
umoral or cellular factors. Syngeneic recipient rats were injected (in
traperitoneally with 4 x 10(7) cells (precultured overnight with eithe
r HDM or bovine serum albumin [BSA]) from lymph nodes of sensitized or
control rats, respectively. Other groups received a tail-vein injecti
on of serum from either HDM-sensitized or control rats. Antigen challe
nge in rats injected with sensitized cells caused increases in pulmona
ry inflammation and in AHR, but no changes in immediate bronchoconstri
ction as compared with control recipients. Antigen challenge in serum
recipients resulted in immediate bronchoconstriction but had no effect
on AHR or on pulmonary inflammation. These data show that immune-medi
ated lung inflammation and AHR are promoted by antigen-specific lympho
cytes, whereas immediate allergic responses are caused by serum factor
s.