The relationship between clinical, serological and bacteriological dia
gnostic criteria have been determined in Brucella ovis culture-positiv
e and Brucella free control rams and also in 110 field rams belonging
to 13 different flocks, in which an epidemiological survey was conduct
ed. In control rams, a direct relationship was found among the existen
ce of positive bacteriological results and the presence of a positive
serological response in the gel diffusion (GD) and complement fixation
(CF) tests for B. ovis. In field rams, the presence of macroscopic te
sticular alterations (MTA) caused by B. ovis was correlated with bacte
riological and serological results. The percentage of reactors and the
percentage of rams showing MTA was not related to the size of flocks
nor to the ratios of ram/ewes. The prevalence of B. ovis infection was
significantly higher in rams from imported breeds than in those from
local ones. Moreover, the younger and the oldest rams showed the lowes
t prevalence of B. ovis infection, but the age of rams did not influen
ce the prevalence of MTA not caused by B. ovis. (C) 1998 Elsevier Scie
nce B.V. All rights reserved.