MINOR GROOVE-BINDING ARCHITECTURAL PROTEINS - STRUCTURE, FUNCTION, AND DNA RECOGNITION

To date, high-resolution structures have been solved for five differen t architectural proteins complexed to their DNA target sites. These in clude TATA-box-binding protein, integration host factor (IHF), high mo bility group I(Y)[HMG I(Y)], and the HMG-box-containing proteins SRY a nd LEF-1. Each of these proteins interacts with DNA exclusively throug h minor groove contacts and alters DNA conformation. This paper review s the structural features of these complexes and the roles they play i n facilitating assembly of higher-order protein-DNA complexes and disc usses elements that contribute to sequence-specific recognition and co nformational changes.