S. Carrere et al., ERG PROTEINS, TRANSCRIPTION FACTORS OF THE ETS FAMILY, FORM HOMO, HETERODIMERS AND TERNARY COMPLEXES VIA 2 DISTINCT DOMAINS, Oncogene, 16(25), 1998, pp. 3261-3268
The ets genes family encodes a group of proteins which function as tra
nscription factors under physiological conditions. We report here that
the Erg proteins, members of the Ets family, form homo and heterodime
ric complexes in vitro. We demonstrate that the Ergp55 protein isoform
forms dimers,vith itself and with the two other isoforms, Ergp49 and
Ergp38. Using a set of Erg protein deletion mutants, we define two dis
tinct domains independently involved in dimerization. The first one is
located in the amino-terminal part of the protein containing the poin
ted domain (PNT), conserved in a subset of Ets proteins. The second on
e resides within the ETS domain, the DNA-binding domain. We also show
that the Erg protein central region behaves as an inhibitory domain of
dimerization and its removal enhances the Ergp55 transactivation prop
erties. Furthermore, Ergp55 forms heterodimers with some other Ets pro
teins. Among the latter, we show that Fli-1, Ets-2, Er81 and Pu-1 phys
ically interact with Erg. Finally, rye show that the formation of the
previously described ternary complex Ergp55/Fos/jun is mediated by ETS
domain and Jun protein, while the ternary complex Ergp49/Fos/Jun is m
ediated by Fos protein.