Many processes, cell motility being on example, require cells to remod
el the actin cytoskeleton in response to both intracellular and extrac
ellular signals. Reorganization of the actin cytoskeleton involves the
rapid disassembly and reassembly of actin filaments, a phenomenon reg
ulated by the action of particular actin-binding proteins. In recent y
ears, an interest in studying actin regulation in unicellular organism
s has arisen. Parasitic protozoan are among these organisms and studie
s of the cytoskeleton functions of these protozoon ore relevant relate
d to either cell biology or pathogenicity To discuss recent data in th
is field, a symposium concerning <<Actin and actin-binding proteins in
protists>> was held on May 8-11 in Paris, France, during the XXXV mee
ting of the French Society of Protistology. As a brief summary of the
symposium we report here findings concerning the in vitro actin dynami
c assembly, as well as the characterization of several actin-binding p
roteins from the parasitic protozoan Entamoeba histolytica, Thrichomon
as vaginalis and Plasmodium knowlesi. In addition, localization of act
in in nonpathogen protists such as Prorocentrum micans and Crypthecodi
nium cohnii is also presented. The data show that some actin-binding p
roteins facilitate organization of filaments into higher order structu
res as pseudopods, while others have regulatory functions, indicating
very particular roles for actin-binding proteins. One of the proteins
discussed during the symposium, the actin depolymerizing factor ADF, w
as shown to enhance the treadmilling rate of actin filaments. in vitro
, ADF binds to the ADP-bound forms of G-actin and F-actin, thereby par
ticipating in and changing the rate of actin assembly. Biochemical app
roaches allowed the identification of a protein complex formed by HSP/
C70-cap32-34 which might also be involved in depolymerization of F-act
in in P. knowlesi. Molecular and cellular approaches were used to iden
tify proteins such as ABP-120 and myosin IB at the leading edge of E.
histolytica. ABP-120 organizes F-actin in a network and myosin IB part
icipates in the pseudopod formation. Similar approaches using T. vagin
alis resulted in the discovery of on actin-binding protein that partic
ipate in the F-actin reorganization during adhesion of parasites to ta
rget cells. This protein is homologous to alpha-actinin from other euk
aryotic cells. Finally, by using cell biology approaches, F-actin was
observed in the cytoplasm as well as in the nucleus of Dinoflagelates.
The recent developments in the molecular genetics of protozoa will pr
ovide new insights to understand the roles of actin-binding proteins d
uring cytoskeleton activities.