TRUE ACROSOME REACTION IN BUFFALO (BUBALUS-BUBALIS) SPERMATOZOA

True acrosome reaction in buffalo spermatozoa using TALP medium, was s tudied. This reaction was estimated using dual-staining procedure. Spe rm per cent motility, true and false acrosome reactions were evaluated each hour during 8 hi of incubation. The per cent motility decreased progressively with increasing time of incubation. The initiation of tr ue acrosome reaction was at 3 hr of the incubation and progressively i ncreased with increasing time of incubation with maximum (60%) at 8 hr . The 3 stages of acrosome reaction such as acrosome swelling. vesicul ation and shedding were observed, The biological significance of the t rue and false acrosome reaction has been discussed.