DECOLORIZATION OF PULP-MILL EFFLUENTS WITH IMMOBILIZED LIGNIN AND MANGANESE PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM

The immobilization of lignin and manganese peroxidase produced by Phan erochaete chrysosporium on Amberlite IRA-400 resin and its utilization on the remediation of effluents from the pulp and paper industry was evaluated. The ion exchange resin shows great efficiency for the enzym e immobilization, allowing almost total enzymatic activity retention i n a short period at (30 min). The immobilized enzyme was very efficien t to removing colour and phenolic species from Kraft El effluent with insignificant adsorption of coloured species by the support. Effluent decolorization of over 50% for 3 h of enzymatic treatment was obtained . For bleaching mixture effluent and black liquor, substantial decolor ization ratios were also observed. Unfortunately, a considerable fract ion of this decolorization was due to adsorption on the resin surface.