EFFECT OF BETA-CYCLODEXTRIN AND ORGANIC MODIFIER ON RESOLUTION OF NITRO-POLYCYCLIC AROMATIC-HYDROCARBONS IN MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Micellar electrokinetic chromatography with a photodiode array detecto r has been employed for the separation of nitro-polycyclic aromatic hy drocarbons, including 2-nitro-fluorene, 9-nitro-anthracene, 3-nitro-fl uoranthene and 1-nitro-pyrene. Sodium deoxycholate was used as a micel le-forming reagent. The effects of beta-cyclodextrin and the organic m odifiers, methanol, acetonitrile and dimethylformamide, on the resolut ion of the nitro-polycyclic aromatic hydrocarbons were studied. Aceton itrile and dimethylformamide were useful modifiers to separate highly hydrophobic nitro-polycyclic aromatic hydrocarbons. Structure isomers, 3-nitro-fluoranthene and 1-nitro-pyrene, were not separated using bet a-cyclodextrin as modifier. The influence of temperature on the resolu tion of the nitro-polycyclic aromatic hydrocarbons were also investiga ted.