USE OF MONOCLONAL-ANTIBODIES AGAINST CHICKEN COCCIDIA TO STUDY INVASION AND EARLY DEVELOPMENT OF EIMERIA-GRUIS IN THE FLORIDA SANDHILL CRANE (GRUS-CANADENSIS)

Eimeria gruis and E. reichenowi are common coccidial parasites of a nu mber of crane species. In the present study, monoclonal antibodies (Mc Abs), elicited against Eimeria spp. of chickens and turkeys, cross-rea cted with sporozoites and developmental stages of E. gruis in the tiss ues of Florida sandhill cranes. These McAbs were used to define the ar ea of the intestine that was invaded by sporozoites of E. gruis and to demonstrate the feasibility of using McAbs to study the early develop ment of E. gruis in the intestines and visceral organs of cranes. At 6 hr postinoculation (PI), E. gruis sporozoites were found primarily fr om just proximal to Meckle's diverticulum in the jejunum to the ileoce cal juncture. Fewer sporozoites were found in the ceca and rectum, and none were found in the duodenum. Most of the sporozoites were in the middle third of the villi and within the lamina propria. At 14 days PI , developmental stages were detected in the ceca, jejunum, liver, and lungs but not in the heart, kidney, or brain. In the ceca and jejunum, the number, location, and maturity of the stages differed markedly.