ARTIFICIAL IMITATION OF GLUTATHIONE-PEROXIDASE WITH 6-SELENIUM-BRIDGED BETA-CYCLODEXTRIN

On the basis of cyclodextrin, 6-selenium bridged beta-cyclodextrin (6- beta-CD-Se-Se-beta-CD, known as 6-SeCD) was synthesized by the selecti ve tosylation of beta-cyclodextrin and nucleophilic displacement by so dium hydroselenide to imitate glutathione peroxidase (GPX). The GPX ac tivity of diselenide 6-SeCD is 4.3 times that of PZ51. The structure o f the mimic 6-SeCD was characterized by means of laser mass spectrosco py, elemental analysis, IR and H-1 NMR. The selenium content and its v alence in 6-SeCD were determined by means of X-ray photoelectron spect ra. Kinetics of the mimic showed that its enzymatic behavior was simil ar to that of native GPX. (C) 1998 Academic Press.