LONG-TERM POSTMORTEM SURVIVAL OF MITOCHONDRIAL GENOMES IN MOUSE SYNAPTOSOMES AND THEIR RESCUE IN A MITOCHONDRIAL DNA-LESS MOUSE-CELL LINE

Citation
S. Ito et al., LONG-TERM POSTMORTEM SURVIVAL OF MITOCHONDRIAL GENOMES IN MOUSE SYNAPTOSOMES AND THEIR RESCUE IN A MITOCHONDRIAL DNA-LESS MOUSE-CELL LINE, Biochemical and biophysical research communications, 247(2), 1998, pp. 432-435
Citations number
19
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
247
Issue
2
Year of publication
1998
Pages
432 - 435
Database
ISI
SICI code
0006-291X(1998)247:2<432:LPSOMG>2.0.ZU;2-P
Abstract
Mitochondrial DNA (mtDNA) transfer was carried out from postmortem mou se tissues to mouse mtDNA-less (rho(0)) cells to determine how long it takes for autolysis of mtDNA after death and whether mtDNA in postmor tem tissues can recover its function in rho(0) cells. The results show ed that mtDNA was stable in postmitotic tissues stored at 4 degrees C. Moreover, mtDNA in postmortem brain tissues stored for up to 1 month still retained functional properties, causing complete recovery of mit ochondrial respiratory function, when it was transferred to rho(0) cel ls. These observations suggest that mtDNA in brain tissue can survive for 1 month after death and can start replication and gene expression in rho(0) cells without showing any functional defects. This procedure might be applied to human autopsy brain tissues for examination of th e influence of accumulated somatic mutations in mtDNA from aged subjec ts. (C) 1998 Academic Press.