ENGINEERING THE LUXCDABE GENES FROM PHOTORHABDUS-LUMINESCENS TO PROVIDE A BIOLUMINESCENT REPORTER FOR CONSTITUTIVE AND PROMOTER PROBE PLASMIDS AND MINI-TN5 CONSTRUCTS
Mk. Winson et al., ENGINEERING THE LUXCDABE GENES FROM PHOTORHABDUS-LUMINESCENS TO PROVIDE A BIOLUMINESCENT REPORTER FOR CONSTITUTIVE AND PROMOTER PROBE PLASMIDS AND MINI-TN5 CONSTRUCTS, FEMS microbiology letters, 163(2), 1998, pp. 193-202
The luxCDABE operon of Photorhabdus luminescens has been cloned and en
gineered as an easily mobilisable cassette flanked by sites for common
ly used restriction enzymes. Constitutive and promoter probe plasmids
utilising the P. luminescens luxCDABE have been constructed using a nu
mber of compatible replicons and antibiotic markers. Complementary to
these plasmids, a range of promoterless and constitutive luxCDABE mini
-Tn5 derivatives has been constructed. The potential of coupling mini-
Tn5 luxCDABE promoter probe transposons with automated luminometry and
photometry to screen for mutants that exhibit growth phase variation
in gene expression is demonstrated. (C) 1998 Federation of European Mi
crobiological Societies. Published by Elsevier Science B.V. All rights
reserved.