MICROSATELLITES IN THE MITOCHONDRIAL GENOME OF PHYTOPHTHORA-CINNAMOMIFAILED TO PROVIDE HIGHLY POLYMORPHIC MARKERS FOR POPULATION-GENETICS

Microsatellites were evaluated as genetic markers for the mitochondria l genome (mtDNA) of Phytophthora cinnamomi for population studies. Two (A)(n) microsatellite loci were cloned from the mtDNA of P. cinnamomi . Amplification products from these loci showed little polymorphism am ong Phytophthora isolates due to their location in coding regions of m tDNA, A further three (A)(n) microsatellite loci obtained from the com plete mtDNA sequence of P. infestans were also not highly polymorphic, although located in non-coding mtDNA. The presence of the (A)(n) micr osatellites was not conserved in the genus Phytophthora. Unlike those of the chloroplast genome of plants, (A)(n) microsatellite loci of mtD NA do not have potential as highly polymorphic markers in Phytophthora . (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B,V. All rights reserved.