Mp. Dobrowolski et al., MICROSATELLITES IN THE MITOCHONDRIAL GENOME OF PHYTOPHTHORA-CINNAMOMIFAILED TO PROVIDE HIGHLY POLYMORPHIC MARKERS FOR POPULATION-GENETICS, FEMS microbiology letters, 163(2), 1998, pp. 243-248
Microsatellites were evaluated as genetic markers for the mitochondria
l genome (mtDNA) of Phytophthora cinnamomi for population studies. Two
(A)(n) microsatellite loci were cloned from the mtDNA of P. cinnamomi
. Amplification products from these loci showed little polymorphism am
ong Phytophthora isolates due to their location in coding regions of m
tDNA, A further three (A)(n) microsatellite loci obtained from the com
plete mtDNA sequence of P. infestans were also not highly polymorphic,
although located in non-coding mtDNA. The presence of the (A)(n) micr
osatellites was not conserved in the genus Phytophthora. Unlike those
of the chloroplast genome of plants, (A)(n) microsatellite loci of mtD
NA do not have potential as highly polymorphic markers in Phytophthora
. (C) 1998 Federation of European Microbiological Societies. Published
by Elsevier Science B,V. All rights reserved.