CLONING, EXPRESSION PATTERN, AND CHROMOSOMAL ASSIGNMENT TO 16Q23 OF THE HUMAN GAMMA-ADAPTIN GENE (ADTG)

Adaptins are important components of clathrin-coated vesicles transpor ting ligand-receptor complexes from the plasma membrane or from the tr ans-Golgi network to lysosomes. Adaptins, together with medium and sma ll subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. We present the cloning and sequencing of the human gamma-adaptin cDNA (HGMW-approved symbol ADTG) consisting of 3723 bp with an open readin g frame capable of encoding a protein of 825 amino acids, 98.9% identi cal to the mouse protein. Northern blot analysis of the mouse and huma n gamma-adaptin genes revealed a ubiquitous and abundant expression, e xcept in human adult lung. Using a monochromosomal somatic cell hybrid panel and fluorescence in situ hybridization, we mapped this gene to human chromosome 16q23, which is syntenic with mouse chromosome 8, ban d D. In addition, we localized genes fbr two other components of the A P-1 adaptor, i.e., the medium (AP47) and small (AP19) subunits, to chr omosomes 19 and 7, respectively. Expression analysis of these genes in human tissues revealed ubiquitously expressed transcripts of approxim ately 2.5 and 1.5 kb, respectively. (C) 1998 Academic Press.