P-31 NMR-STUDY OF THE INTERACTIONS BETWEEN OLIGODEOXYNUCLEOTIDES CONTAINING (6-4)PHOTOPRODUCT AND FAB FRAGMENTS OF MONOCLONAL-ANTIBODIES SPECIFIC FOR (6-4)PHOTOPRODUCT

Authors
TORIZAWA T KATO K KIMURA Y ASADA T KOBAYASHI H KOMATSU Y MORIOKA H NIKAIDO O OHTSUKA E SHIMADA I
Citation
T. Torizawa et al., P-31 NMR-STUDY OF THE INTERACTIONS BETWEEN OLIGODEOXYNUCLEOTIDES CONTAINING (6-4)PHOTOPRODUCT AND FAB FRAGMENTS OF MONOCLONAL-ANTIBODIES SPECIFIC FOR (6-4)PHOTOPRODUCT, FEBS letters, 429(2), 1998, pp. 157-161
Citations number
46
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
429
Issue
2
Year of publication
1998
Pages
157 - 161
Database
ISI
SICI code
0014-5793(1998)429:2<157:PNOTIB>2.0.ZU;2-2
Abstract
A P-31 nuclear magnetic resonance (NMR) study of the interactions betw een oligonucleotides containing the (6-4) photoproduct and the Fab fra gments of monoclonal antibodies (64M3 and 64M5) recognizing the (6-4) photoproduct is reported. The P-31 chemical shift data indicate that b ackbone conformation of (6-4) adduct is affected by the presence of fl anking oligodeoxynucleotides, and (6-4) adducts with different backbon e conformations are accommodated in the antigen binding sites of these antibodies. It was also revealed that epitopes for these antibodies c onsist of not only the (6-4) adduct but the flanking di- or tri-deoxyn ucleotides on both the 5' and 3' sides as well. (C) 1998 Federation of European Biochemical Societies.