ISOLATION OF CDNA LIBRARIES FROM INDIVIDUAL HUMAN PREIMPLANTATION EMBRYOS

Citation
Y. Verlinsky et al., ISOLATION OF CDNA LIBRARIES FROM INDIVIDUAL HUMAN PREIMPLANTATION EMBRYOS, Molecular human reproduction (Print), 4(6), 1998, pp. 571-575
Citations number
28
Categorie Soggetti
Reproductive Biology","Developmental Biology
ISSN journal
13609947
Volume
4
Issue
6
Year of publication
1998
Pages
571 - 575
Database
ISI
SICI code
1360-9947(1998)4:6<571:IOCLFI>2.0.ZU;2-Y
Abstract
Although available data from the mouse model suggest that morphogenesi s during preimplantation development is dependent on the expression of embryonic genes, convincing data for human preimplantation embryos ar e missing. To investigate the expression of genes involved in human pr eimplantation development we constructed cDNA libraries from human ind ividual blastocysts and screened them for the expression of beta-actin , Coss, homeoboxes OCT-3 and HOXA4, and HLA-G and hMLH-1 genes, beta-a ctin, Coss, and OCT-3 were detected by reverse transcription-polymeras e chain reaction (RT-PCR), while HOXA4, HLA-G and hMLH-1 were undetect ed. Sequencing of 48 random clones from two libraries revealed a diffe rent identity to the known genes including 99% identity to human histo ne 3.1 and human ribosome protein S25 complete cDNA. These data demons trate the feasibility of constructing cDNA libraries from individual h uman preimplantation embryos and their potential usefulness in the ass essment of the relevance of specific gene expression in the failures o f preimplantation development.