Y. Verlinsky et al., ISOLATION OF CDNA LIBRARIES FROM INDIVIDUAL HUMAN PREIMPLANTATION EMBRYOS, Molecular human reproduction (Print), 4(6), 1998, pp. 571-575
Although available data from the mouse model suggest that morphogenesi
s during preimplantation development is dependent on the expression of
embryonic genes, convincing data for human preimplantation embryos ar
e missing. To investigate the expression of genes involved in human pr
eimplantation development we constructed cDNA libraries from human ind
ividual blastocysts and screened them for the expression of beta-actin
, Coss, homeoboxes OCT-3 and HOXA4, and HLA-G and hMLH-1 genes, beta-a
ctin, Coss, and OCT-3 were detected by reverse transcription-polymeras
e chain reaction (RT-PCR), while HOXA4, HLA-G and hMLH-1 were undetect
ed. Sequencing of 48 random clones from two libraries revealed a diffe
rent identity to the known genes including 99% identity to human histo
ne 3.1 and human ribosome protein S25 complete cDNA. These data demons
trate the feasibility of constructing cDNA libraries from individual h
uman preimplantation embryos and their potential usefulness in the ass
essment of the relevance of specific gene expression in the failures o
f preimplantation development.