PKN INTERACTS WITH A PARANEOPLASTIC CEREBELLAR DEGENERATION-ASSOCIATED ANTIGEN, WHICH IS A POTENTIAL TRANSCRIPTION FACTOR

PKN is a fatty acid-activated serine/threonine protein kinase, having a catalytic domain homologous to protein kinase C family. PKN has been recently reported to interact with a small GTP-binding protein Rho an d cytoskeletal proteins such as neurofilament and alpha-actinin. To id entify the new components of the PKN-signaling pathway, the yeast two- hybrid system was employed. Using the amino-terminal regulatory domain of PKN as a bait, cDNA encoding a neural antigen PCD17, which is reco gnized by characteristic antibodies of patients with paraneoplastic ce rebellar degeneration, was isolated from a human brain cDNA library. T he interaction between PKN and PCD17 was also determined by the in vit ro binding analysis. PCD17 was coimmunoprecipitated with PKN from the lysate of COS7 cells transfected with both expression constructs for P KN and the amino-terminal region of PCD17. PCD17 was phosphorylated by PKN, and the extent of this phosphorylation was enhanced by addition of 40 mu M arachidonic acid. The amino-terminal region of PCD17 could form a homodimer in vitro, and PCD17 fused to the Gal4 DNA binding dom ain showed the transcriptional transactivation of the chloramphenicol acetyltransferase reporter gene linked to 5 Gal4 binding sites and min imal promoter in rat C6 glioma cells. These results suggest the partic ipation of PCD17 in gene expression and lead to a clue for elucidating the PKN signaling pathway from the cytosol to the nucleus. (C) 1998 A cademic Press.