J. Russell et al., PRETREATMENT WITH POLYNITROXYL ALBUMIN (PNA) INHIBITS ISCHEMIA-REPERFUSION INDUCED LEUKOCYTE-ENDOTHELIAL CELL-ADHESION, Free radical biology & medicine, 25(2), 1998, pp. 153-159
Recently published evidence indicates that polynitroxylated albumin (P
NA) protects tissues against ischemia/reperfusion (I/R) injury, possib
ly by enhancing tissue redox activity. The objective of this study was
to determine if PNA treatment alters the leukocyte-endothelial cell a
dhesion that is normally elicited by I/R. PNA, human serum albumin (HS
A) or saline were administered (i.v.) 5 min before reperfusion. Venula
r diameter, red blood cell velocity, wall shear rate, systemic hematoc
rit, systemic arterial pressure, as well as the number of adherent and
emigrated leukocytes were monitored in rat mesenteric venules before
and after 20 min of ischemia and 30 min of reperfusion. In saline-trea
ted rats, I/R elicited a 5.3-fold increase in leukocyte adhesion and a
1.8-fold increase in leukocyte emigration. HSA-treated animals exhibi
ted 4.0 and 2.3-fold increases in leukocyte adherence and emigration,
respectively. In PNA-treated rats, the number of adherent leukocytes i
ncreased only 2.1-fold increase in adherent leukocytes, while leukocyt
e emigration was completely inhibited. The PNA-induced attenuation of
leukocyte adherence/emigration could not be attributed to alterations
in systemic or local hemodynamics (red blood cell velocity or wall she
ar rate). PNA was also shown to be a potent inhibitor of xanthine-xant
hine oxidase mediated adhesion of human neutrophils to cultured human
endothelial cells. These findings indicate that PNA may protect tissue
s against I/R injury by attenuating leukocyte-endothelial cell adhesio
n. (C) 1998 Elsevier Science Inc.