COMPARISON OF A MULTIPORE COLUMN WITH A MIXED-BED COLUMN FOR SIZE-EXCLUSION CHROMATOGRAPHY

A new type of column packed with polystyrene gels which have a broad p ore size distribution in a particle thenceforth referred to as the mul tipore column) was developed, and its performance was compared with th e so-called linear column thenceforth referred to as the mixed-bed col umn). The chromatogram of the epoxy resin (Epikote 1009) on the multip ore column showed a monomodal distribution, but the chromatogram on th e mixed-bed column showed the inflection on the chromatogram at the mo lecular mass of about 10,000 as polystyrene equivalent. There was a bi g difference in the pore size distributions of gels packed in the two types of columns; the pore size distribution of the multipore column s howed the trapezoid, while that of the mixed-bed column had sharp maxi ma for pores with a diameter of 0.08 mu m. This difference in the pore size distribution may be the main reason for the inflection phenomeno n, though the: calibration curves of these two types of columns were a lmost the same, The multipore column also had the advantage of the mix ed-bed column in the linearity of the calibration curve.