CHANGES IN IP(3)R1 AND SERCA2B MESSENGER-RNA LEVELS IN THE GERBIL BRAIN AFTER CHRONIC ETHANOL ADMINISTRATION AND TRANSIENT CEREBRAL ISCHEMIA-REPERFUSION
Jm. Xia et al., CHANGES IN IP(3)R1 AND SERCA2B MESSENGER-RNA LEVELS IN THE GERBIL BRAIN AFTER CHRONIC ETHANOL ADMINISTRATION AND TRANSIENT CEREBRAL ISCHEMIA-REPERFUSION, Molecular brain research, 56(1-2), 1998, pp. 22-28
Despite epidemiological studies indicating a positive relationship bet
ween alcohol and stroke, little is known with regard to effect of chro
nic alcohol on neuronal injury after stroke. In this study, we examine
d the effect of chronic ethanol on mRNA levels of sarcoplasmic or endo
plasmic Ca2+-ATPase (SERCA2b) and inositol 1,4,5-triphosphate receptor
(IP(3)R1) in gerbils subjected to global cerebral ischemia induced by
ligation of both common carotid arteries. Gerbils were given daily by
intragastric intubation either a liquid diet containing ethanol (4 g/
kg) or the same diet with an isocaloric amount of sucrose for 35 days.
They were subsequently subjected to a 5 min ischemic insult followed
by reperfusion for 48 h. In agreement with other studies, ischemic ins
ult caused significant decreases (P < 0.05) in mRNA levels of both IP(
3)R1 and SERCA2b in the hippocampal CA1 region but not in the dentate
gyrus. Nevertheless, despite a significant (P < 0.05) decrease in SERC
A2b mRNA in the Purkinje neurons, chronic ethanol did not alter the ex
pression of this mRNA species in the hippocampal CA1 neurons nor did i
t alter the decrease in SERCA2b mRNA due to cerebral ischemic insult.
Since IP(3)R1 and SERCA2b are key mediators for regulation of intracel
lular Ca2+ stores, the decrease in SERCA2b mRNA but not IP(3)R1 mRNA i
n cerebellar neurons may be an important mechanism underlying alterati
on of calcium homeostasis and cerebellar degeneration upon chronic eth
anol consumption. (C) 1998 Elsevier Science B.V.