Pj. Valentine et al., IDENTIFICATION OF 3 HIGHLY ATTENUATED SALMONELLA-TYPHIMURIUM MUTANTS THAT ARE MORE IMMUNOGENIC AND PROTECTIVE IN MICE THAN A PROTOTYPICAL AROA MUTANT, Infection and immunity, 66(7), 1998, pp. 3378-3383
A panel of Salmonella typhimurium 14028s mutants, which were previousl
y shown to be highly attenuated in the BALB/c mouse model of infection
, were analyzed for their potential as live Salmonella oral-vaccine ca
ndidates. A prototypical aroA mutant was chosen as a basis of comparis
on. From the panel of mutants initially chosen for this study, three m
utants with comparable levels of attenuation elicited higher Salmonell
a-specific serum immunoglobulin G (IgG) and/or mucosal secretory-IgA a
ntibody titers than the aroA vaccine strain. The three mutants, CL288,
CL401, and CL554, also elicited a better protective immune response t
han the aroA control strain, after a single oral dose of 1 x 10(9) to
2 x 10(9) bacteria.