ACTIVITY OF SOME ENZYMES IN THE BLOOD-PLASMA OF HYLLA RABBITS FED VARIOUS PROPORTIONS OF SUBSTRATE FROM THE PRODUCTION OF THE PLEUROTUS-PULMONARIUS MUSHROOM

The nutritive effect of different amounts of substrate from the produc tion of Pleurotus pulmonarius on the activity of AST, ALT, LD, CK, GGT and AP in the blood plasma of rabbits, after a nine weeks experimenta l fattening, was investigated. Sixty male Hyla rabbits, divided in fou r groups equal in number, one control (C) and three experimental group s (E-1, E-2' E-3). were included in experiment. The control group (C) was fed the standard fodder mix without substrate. Added to the standa rd mix, the groups E-1, E-2 and E-3 received various proportions, 10%, 20%, and 30%, respectively, of the substrate left from the production of Pleurotus pulmonarius. Different proportions of added substrate di d not affect the activity of AST and GGT, respectively, whose values ( x +/- s.d,) in the control group (C) amounted to 32.49 +/- 12.8 U.l(-1 ) and 6.14 +/- 1.35 U.l(-1), respectively. At the same time the activi ty of ALT was altered by experimental diets. The activity of ALT in th e control group (C) amounted to 28.0 +/- 8.81 U.l(-1), whereas in the E-3 group it was significantly greater (P<0.05) than in the group C an d E-2, i,e. 38.86 +/- 11.95 U.l(-1): Under the conditions of experimen tal feeding the activity of AP was greater too: in the control group ( C) it amounted to 107 +/- 42.5 U.l(-1), but in the group E-3 it was si gnificantly greater (P<0.05) and amounted to 158.7 +/- 27.7 U.l-(1.) O n the contrary, the activity of CK is decreased during the experimenta l feeding (P<0.07), and In the control group (C) it amounted to 2044 /- 647 U.l(-1), in the E-3 group to 1479.6 +/- 927 U.l(-1). The activi ty of lactate dehydrogenase (LD) generally reflected the composition o f fattening diets and is inversely proportionate to the share of subst rate added. The highest values of LD activity was found in group C (94 .57 +/- 24.78 U.l(-1)), and the lowest was in group E-3 (63.49 +/- 14. 12 U.l(-1)). The difference was significant compared to the C and E-1, groups (P<0.01) and to the E-2 (P<0.05) group. The results indicate B -1 hypovitaminosis and the development of compensatory metabolic acido sis.