HILL AND SCATCHARD PLOTS OF DAPHNIA-MAGNA HEMOGLOBIN

The oxygen equilibrium curve of Daphnia magna hemoglobin (Hb) was meas ured accurately with an automatic recording apparatus. According to an Adair's octomerous equation, eight Adair's constants were determined by curve fitting. The Hill and Scatchard plots for binding of oxygen ( O-2) to Daphnia Hb were performed and their slopes calculated. The Hil l plot of Daphnia Hb was characterized by a double-peak-like pattern. These results were compared to those of human Hb, and the O-2 binding characteristics of Daphnia Hb discussed.