ACCLIMATION TEMPERATURE AFFECTS THE FUNCTIONAL AND STRUCTURAL-PROPERTIES OF LACTATE-DEHYDROGENASE FROM FISH (MISGURNUS-FOSSILIS) SKELETAL-MUSCLES

Acclimation of leach to low and high temperatures gives rise to certai n changes in properties of M(4)-LDH from skeletal muscles. The apparen t K-m values for pyruvate of the enzyme purified from fish adapted to low temperatures shows a minimum at low measurement temperatures, wher eas adaptation to high temperatures leads to the enzyme showing a mini mum at high temperatures. The M(4)-LDH purified from fish acclimated t o low temperatures reveals greater stability both to thermal and urea- induced inactivation. The differences in the kinetic properties of LDH from fish acclimated to low and high temperatures disappeared after t reatment with urea and subsequent reactivation.