Cc. Ku et al., PURIFICATION AND IMMUNOLOGICAL CHARACTERIZATION OF COLOR CARP (CYPRINUS-CARPIO) FIBROBLAST HEAT-SHOCK PROTEINS, Comparative biochemistry and physiology. B. Comparative biochemistry, 107(1), 1994, pp. 147-159
Eighty-seven, 72 and 30 kDa heat-shock proteins from color carp testis
cell line, CCT, were purified, and rabbit antibodies were raised agai
nst them. After heat shock at 37 degrees C, hsp87 appeared mainly in t
he cytoplasm and hsp72 and hsp30 appeared in the nucleus. When cells w
ere restressed at 37 degrees C for 8 hr after three recovery periods,
the intensity and localization of the three anti-CCT hsps staining cha
nged from those following an initial stress for 8 hr. The anti-CCT hsp
87 and hsp72 antibodies crossreacted with proteins of similar molecula
r weights in all tested fish, lizard, mouse and human cell lines, but
they showed various degrees in antigenic relevance.