PURIFICATION AND IMMUNOLOGICAL CHARACTERIZATION OF COLOR CARP (CYPRINUS-CARPIO) FIBROBLAST HEAT-SHOCK PROTEINS

Eighty-seven, 72 and 30 kDa heat-shock proteins from color carp testis cell line, CCT, were purified, and rabbit antibodies were raised agai nst them. After heat shock at 37 degrees C, hsp87 appeared mainly in t he cytoplasm and hsp72 and hsp30 appeared in the nucleus. When cells w ere restressed at 37 degrees C for 8 hr after three recovery periods, the intensity and localization of the three anti-CCT hsps staining cha nged from those following an initial stress for 8 hr. The anti-CCT hsp 87 and hsp72 antibodies crossreacted with proteins of similar molecula r weights in all tested fish, lizard, mouse and human cell lines, but they showed various degrees in antigenic relevance.