Wg. Zijlstra et al., SPECTROPHOTOMETRY OF HEMOGLOBIN - ABSORPTION-SPECTRA OF RAT OXYHEMOGLOBIN, DEOXYHEMOGLOBIN, CARBOXYHEMOGLOBIN, AND METHEMOGLOBIN, Comparative biochemistry and physiology. B. Comparative biochemistry, 107(1), 1994, pp. 161-166
The absorptivity at 540 nm of methemoglobincyanide from rat blood was
determined on the basis of iron and found to be equal to the establish
ed value for human methemoglobincyanide (11,01/mmol/cm). On this basis
the absorption spectra of the common derivatives were determined for
rat hemoglobin. Preparation of clear, concentrated solutions of the pu
re hemoglobin species was hampered by the low solubility and the tende
ncy to spontaneous methemoglobin formation of rat hemoglobin. As for t
he wavelengths of maximum and minimum absorption there was little diff
erence between rat and man. As for the absorptivities there were signi
ficant differences, especially in the methemoglobin spectrum. These di
fferences should be taken into account in spectrophotometric multicomp
onent analysis.