IDENTIFICATION AND CDNA CLONING OF NOVEL JUVENILE-HORMONE RESPONSIVE GENES FROM FAT-BODY OF THE BEAN BUG, RIPTORTUS-CLAVATUS BY MESSENGER-RNA DIFFERENTIAL DISPLAY
M. Hirai et al., IDENTIFICATION AND CDNA CLONING OF NOVEL JUVENILE-HORMONE RESPONSIVE GENES FROM FAT-BODY OF THE BEAN BUG, RIPTORTUS-CLAVATUS BY MESSENGER-RNA DIFFERENTIAL DISPLAY, Insect biochemistry and molecular biology, 28(3), 1998, pp. 181-189
Juvenile hormone (JH) induces termination of diapause and initiation o
f reproductive maturation in the adult female bean bug, Riptortus clav
atus. Applying PCR-based differential display, we have identified four
novel JH-responsive cDNAs, that is, three repressible (JR-I, 2 and 3)
and one inducible (JI-1). These DNA fragments were partially sequence
d and compared with sequences in the data base. JR-3 was shown to have
similarity to the transferrins of other insects, which have been repo
rted as JH-suppressed genes. JI-1 has similarity to vitellogenin of Ae
des aegypti. On the other hand, JR-l and 2 have no significant similar
ity to other known sequences. For JR-I, the;full cDNA sequence was det
ermined: it contained 913 bp, encoding 194 amino acid residues with a
calculated M.W. of 21531 Da in the mature protein. A total of six JH-r
esponsive genes or cDNAs (four suppressible and two inducible or stimu
lated), including the already isolated JH-responsive cyanoprotein gene
s (CP-alpha and beta), have been isolated and are available for furthe
r comparative analysis of gene structure and regulatory mechanisms in
the same tissue under the same hormonal conditions. (C) 1998 Elsevier
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