K. Padmanabhan et al., COMPUTER VISION ANALYSIS OF SOMATIC EMBRYOS OF SWEET-POTATO [IPOMOEA-BATATAS (L.) LAM.] FOR ASSESSING THEIR ABILITY TO CONVERT TO PLANTS, Plant cell reports, 17(9), 1998, pp. 681-684
Apical and axial shoot tips of sweet potato were cultured to produce s
omatic embryos that mature and develop into plants in basal nutrient m
edium. However, the lack of high regeneration efficiency is an impedim
ent to the use of somatic embryos to produce synthetic seeds. Conversi
on experiments with mature embryos over a 20-day period revealed that
80-90% of the embryos formed roots but only 40-50% formed shoots. Usin
g computer vision and canonical or Fisher discriminant function (CDA)
analysis along with conversion results, it was possible to correctly c
lassify competent embryos 40-50% of the time based on size features, 5
0-60% of the time based on shape features, and 55-60% of the time base
d on color features. Non-competent embryos were correctly classified 6
5-75%, 55-60%, and 70-75% of the time based on size, shape, and color,
respectively. These results can be used effectively to identify and s
elect competent embryos for improved regeneration efficiency.