ENZYMATIC-PROPERTIES OF ANIONIC TRYPSINS FROM THE HEPATOPANCREAS OF CRAYFISH, PROCAMBARUS-CLARKII

Maximum amidolytic and esterolytic activities of crayfish hepatopancre atic trypsins occurred in a pH range of 7.5-8.5. Activity of trypsin A increased by addition of 0.5 mM of Ca2+ ions; however, other trypsins were not affected by this concentration. Kinetic properties of crayfi sh trypsins toward esterolytic reaction were similar, but those for am idolytic reaction were different. Activation energies for esterolytic reaction were approximately 6.4-9.0 kcal/mole, while those for amidoly tic reaction were between 5.9 and 6.9 kcal/mole.