SEA BASS (DICENTRARCHUS-LABRAX L) VITELLOGENIN .2. VALIDATION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)

Authors
MANANOS E NUNEZ J ZANUY S CARRILLO M LEMENN F
Citation
E. Mananos et al., SEA BASS (DICENTRARCHUS-LABRAX L) VITELLOGENIN .2. VALIDATION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA), Comparative biochemistry and physiology. B. Comparative biochemistry, 107(2), 1994, pp. 217-223
Citations number
37
Categorie Soggetti
Biology
Journal title
Comparative biochemistry and physiology. B. Comparative biochemistry → ACNP
ISSN journal
03050491
Volume
107
Issue
2
Year of publication
1994
Pages
217 - 223
Database
ISI
SICI code
0305-0491(1994)107:2<217:SB(LV.>2.0.ZU;2-4
Abstract
In this study, a competitive ELISA was developed for the quantificatio n of vitellogenin (VTG) in sea bass. The purity of the antigen (VTG) a nd the specificity of the antibody (AbVTG) used in the assay, were ass essed by ELISA and radial immunodiffusion, and no crossreactivity was observed with the male plasma. The sensitivity range of the ELISA was 1-60 ng/ml (85-20% of binding). The intra- and inter-assay variations (at 50% of binding) were 5.3% (N = 5) and 9.8% (N = 9), respectively. Annual VTG levels were quantified in animals reared under fish farming conditions and the VTG levels were correlated with the oocyte develop ment.