BACTERIAL ERADICATION BY CLINAFLOXACIN, CI-990, AND CIPROFLOXACIN EMPLOYING MBC TEST, IN-VITRO TIME-KILL AND IN-VIVO TIME-KILL STUDIES

Bactericidal activities of clinafloxacin, CI-990 and ciprofloxacin wer e examined and compared for a small collection of Gram-positive and Gr am-negative pathogens. In-vitro antimicrobial activities were measured by MIC, MBC and time-kill studies; in-vivo efficacy of each drug was assessed in an acute lethal mouse infection model that monitored media n protective dose, eradicative dose and time-kill in blood. In general , for all test methods, activity of clinafloxacin exceeded that of CI- 990, which in turn exceeded that of ciprofloxacin. Improvement in clin afloxacin and CI-990 activity over ciprofloxacin was more apparent aga inst Grampositive cocci; differences were less noticeable against Esch erichia coil and Pseudomonas aeruginosa. Clinafloxacin activity was no tably high against Staphylococcus aureus and, against ciprofloxacin-re sistant strains, this high activity was maintained in vitro and measur able activity was demonstrated in vivo.