Nitrous oxide (N2O) may be produced in the soil by both abiotic and bi
otic processes, with the contributions from biotic processes being mor
e significant. The major direct, biotic sources of N2O are denitrifica
tion and nitrification, although nitrifying bacteria also make an indi
rect contribution, because the NO, produced may be subject to denitrif
ication. N2O is both produced and consumed by the process of denitrifi
cation, and the balance between production and consumption determines
net N2O production. In this paper, the biological processes of N2O pro
duction, the approaches to determining the contributions from these di
fferent sources, and the way in which the different interactings proce
sses influence overall N2O emission are outlined. (C) Elsevier, Paris.