INFLUENCE OF HORIZONTAL CLINOSTAT ROTATION ON ROOT-TIP PROTEINS OF VICIA-FABA

Soluble and membrane-bound proteins were SDS-extracted from the root t ip tissue of Vicia faba after 15 h and 24 h of clinostat rotation and compared with the protein pattern, of vertically rotated control plant s. The protein pattern was analysed by SDS-PAGE and Western-immunoblot s with polyclonal antibodies raised to ubiquitin, the ubi-3 extension protein, calmodulin, and monoclonal anti-actin antibodies. All Western -immunoblots were densitometrically analysed. The results showed excep t of the studies with anti-ubiquitin antibodies a decrease in immunore sponding proteins after horizontal clinostat rotation. Contrarily, an actin-related protein of 47 kDa increased. A degradation of these prot eins caused by omnilateral gravistimulation is assumed to represent a stress situation.