CHARACTERIZATION OF VANADATE-SENSITIVE ATPASE ACTIVITY OF PLASMA-MEMBRANE VESICLES PURIFIED BY PHASE PARTITION FROM PEACH-TREE LEAVES

Authors
PETEL G MARQUAT C CHAUBRON F GENDRAUD M
Citation
G. Petel et al., CHARACTERIZATION OF VANADATE-SENSITIVE ATPASE ACTIVITY OF PLASMA-MEMBRANE VESICLES PURIFIED BY PHASE PARTITION FROM PEACH-TREE LEAVES, Plant physiology and biochemistry, 31(5), 1993, pp. 765-771
Citations number
35
Categorie Soggetti
Plant Sciences
Journal title
Plant physiology and biochemistry → ACNP
ISSN journal
09819428
Volume
31
Issue
5
Year of publication
1993
Pages
765 - 771
Database
ISI
SICI code
0981-9428(1993)31:5<765:COVAAO>2.0.ZU;2-Y
Abstract
A pure plasmalemma-enriched fraction was obtained, after aqueous two-p hase partition, from Peach-tree leaves cell. Characterization of the v anadate-sensitive ATPase has shown that plasma membrane ATPase found i n woody plants has similar characteristics as plasmalemma ATPases obse rved in other plant species (vanadate sensitivity, K(m)), but plasmale mma ATPase specific activity was found to be lower than in other speci es. In Peach-tree cells, plasma membrane ATPase was identified as a pe ptide of 110-115 kDa.