NOVEL HUMAN-PAPILLOMAVIRUS (HPV) DNA-SEQUENCES FROM RECURRENT CUTANEOUS AND MUCOSAL LESIONS OF A STOMA-CARRIER

Recent studies have demonstrated a high prevalence of human papillomav irus (HPV) types originally believed to be restricted to patients with epidermodysplasia verruciformis (EV) in benign and malignant skin tum ors of the general population. Other groups detected typical mucosal H PV in skin tumors, We have investigated recurrent leukoplakial cutaneo us and mucosal lesions located around the ileostoma of a woman with ul cerative colitis for the presence of HPV. Cutaneous,mucocutaneous, and mucosal ileostoma-biopsies were analyzed by three different polymeras e chain reaction protocols for genital, cutaneous, and cutaneous EV-as sociated HPV types, Polymerase chain reaction products were cloned, se quenced, and submitted to phylogenetic analyses, HPV-DNA sequences of the EV-HPV group could be detected in all biopsies, whereas genital/mu cosal or cutaneous HPV types were not found. HPV types detected compri sed HPV20, HPV23, HPV38, and four putatively novel HPV types that belo ng to different clusters of the EV-HPV group B1. Different HPV types p revailed in cutaneous, mucocutaneous, and mucosal lesions and the numb er of HPV sequences found per lesion varied between one and three, Our data show the association of recurrent lesions around a stoma and at the ileum with known and novel EV-HPV types. These results emphasize t he plurality of HBV and yield data for the possible transmission of cu taneous NPV to mucosal areas of the intestine.