P. Kelleher et Sc. Knight, IL-12 INCREASES CD80 EXPRESSION AND THE STIMULATORY CAPACITY OF BONE-MARROW-DERIVED DENDRITIC CELLS, International immunology (Print), 10(6), 1998, pp. 749-755
Dendritic cells (DC) are potent antigen-presenting cells derived from
CD34 bone marrow stem cells. They undergo a series of maturational ste
ps that allow them to stimulate primary T cell responses. several cyto
kines are known to contribute to this process. In this study murine DC
maturing from bone marrow progenitors under the influence of granuloc
yte macrophage colony stimulating factor and tumour necrosis factor-cl
were found to produce IL-12 as measured by ELISA and by flow cytometr
y to detect intracellular cytokine. Administration of additional IL-12
from day 3 to 7 of culture altered the function and phenotype of DC;
enhanced stimulation of T cell proliferation by DC in allogeneic mixed
leukocyte reactions was associated with an increase in the surface ex
pression of CD80 on DC. These effects were dose dependent, and were co
nsistently seen with IL-12 at 25 ng/ml and were less marked with IL-12
at 50 ng/ml. These results show that IL-12 is both produced by DC and
can increase their stimulatory capacity. The findings suggest that th
ere may be an autocrine effect of IL-12 on DC maturation and function.