KID-1, A PROTEIN-KINASE INDUCED BY DEPOLARIZATION IN BRAIN

Membrane depolarization leads to changes in gene expression that modul ate neuronal plasticity. Using representational difference analysis, w e have identified a previously undiscovered cDNA,KID-1 (kinase induced by depolarization), that is induced by membrane depolarization or for skolin, but not by neurotrophins or growth factors, in PC12 pheochromo cytoma cells, KID-1 is an immediate early gene that shares a high degr ee of sequence similarity with the family of PIM-1 serine/threonine pr otein kinases, Recombinant KID-1 fusion protein is able to catalyze bo th histone phosphorylation and autophosphorylation, KID-1 mRNA is pres ent in a number of unstimulated tissues, including brain. In response to kainic acid and electroconvulsive shock-induced seizures, KID-1 is induced in specific regions of the hippocampus and cortex.