STUDY OF HPLC RETENTION BEHAVIOR OF 1-HYDROXY-IMIDAZOLE-3-N-OXIDES, SUBSTITUTED IMIDAZOLES, AND IMIDAZOLE DERIVATIVES USING MOLECULAR AND ADSORPTION PARAMETERS

High performance liquid chromatography (HPLC) was applied to study the behavior of a series of 1-hydroxy-imidazole-3-N-oxides (HIO) in solut ion. Some thermodynamic parameters influencing solute retention were d etermined from the temperature dependence of the capacity factor (k). A linear relation was derived between k and molecular parameters consi dering intermolecular association, which was predicted and confirmed. Delta G(ads) values are also highly correlated with connectivity index , molar refractivity and Van der Waals volumes. Retention data for twe nty closely related imidazoles were measured and analyzed in relation to hydrophilic parameters.