NOVEL BRANCHED NOD FACTOR STRUCTURE RESULTS FROM ALPHA-(1-]3) FUCOSYL-TRANSFERASE ACTIVITY - THE MAJOR LIPO-CHITIN OLIGOSACCHARIDES FROM MESORHIZOBIUM-LOTI STRAIN NZP2213 BEAR AN ALPHA-(1-]3) FUCOSYL SUBSTITUENT ON A NONTERMINAL BACKBONE RESIDUE
Mma. Olsthoorn et al., NOVEL BRANCHED NOD FACTOR STRUCTURE RESULTS FROM ALPHA-(1-]3) FUCOSYL-TRANSFERASE ACTIVITY - THE MAJOR LIPO-CHITIN OLIGOSACCHARIDES FROM MESORHIZOBIUM-LOTI STRAIN NZP2213 BEAR AN ALPHA-(1-]3) FUCOSYL SUBSTITUENT ON A NONTERMINAL BACKBONE RESIDUE, Biochemistry, 37(25), 1998, pp. 9024-9032
Mesorhizobium loti has been described as a microsymbiont of plants of
the genus Lotus. Lipochitin oligosaccharides (LCOs), or Nod factors, p
roduced by several representative M. loti strains all have similar str
uctures. Using fast-atom-bombardment tandem mass spectrometry and NMR
spectroscopy, we have now examined the LCOs from the type strain NZP22
13 and observed a much greater variety of structures than has been des
cribed for the strains of M. loti studied previously. Interestingly, w
e have identified as the major LCO a structure that bears a fucose res
idue alpha-1,3-linked to the GlcNAc residue proximal to the nonreducin
g terminal GlcNAc residue. This is the first time, to our knowledge, t
hat substitution on an internal GlcNAc residue of the LCO backbone has
been observed. This novel LCO structure suggests the presence of a no
vel fucosyltransferase activity in strain NZP2213, Since the presence
of this extra structure does not have the effect of broadening the hos
t range, we suggest that the modification of the LCOs with a fucose re
sidue linked to a nonterminal GlcNAc residue might provide protection
against degradation by a particular host plant enzyme (e.g., a chitina
se) or alternatively represents adaptation to a particular host-specif
ic receptor. The action of the alpha-(1-->3) fucosyltransferase seems
to reduce significantly the activity of NodS, the methyltransferase in
volved in the addition of the N-methyl substituent to the nonreducing
terminal GlcNAc residue. An additional novel LCO structure has been id
entified having only a GlcNAc(2) backbone. This is to our knowledge th
e first description of such a minimal LCO structure.