NNK, a tobacco-specific nitrosamine, is a potent lung carcinogen in A/
J nice. One Possible mechanism of reducing NNK-induced lung tumorigene
sis is decreased delivery of NNK to lung as a result of enhanced hepat
ic CYP activity. Pretreatment with I3C, a known CYP inducer, results i
n inhibition of tumor multiplicity, decreased DNA adducts in lung, and
increased DNA adducts in liver, due to induction of hepatic activatio
n of NNK. A more preferable means of inhibition of NNK tumorigenesis i
nvolves direct inhibition of CYP enzymes responsible for NNK activatio
n in lung. The arylalkyl isothiocyanates PEITC, PPITC, PBITC, PPeITC,
and PHITC are effective inhibitors of NNK-induced lung tumorigenicity
and DNA adduction. PEITC inhibits NNK-induced lung tumors at a dose of
5 mu mol/day, but not at doses of 1 or 0.2 mu mol/day. PPITC, PBITC,
PPeITC, and PHITC are considerably more potent inhibitors than PEITC,
resulting in significant reductions in tumor multiplicity at doses of
0.2 mu mol/day. For these compounds, there is a good correlation Betwe
en inhibition of tumor multiplicity and inhibition of pulmonary O-6-me
thylguanine. LIM, previously shown by Wattenberg to be an effective in
hibitor of NNK-induced lung tumors, and other monoterpenes are good in
hibitors of NNK activation in vitro or in vivo. Thus, compounds that m
odulate the metabolic activation of NNK can be potent inhibitors of NN
K tumorigenesis.