As. Mayol et al., CD34(-) SUBSETS - COLLECTION AND ENGRAFTMENT KINETICS IN AUTOLOGOUS PERIPHERAL-BLOOD STEM-CELLS TRANSPLANTATION() CELL DOSE AND CD33(), Haematologica, 83(6), 1998, pp. 489-495
Background and Objective. We analyzed the factors that affected the nu
mber and quality of peripheral blood stem cells (PBSC) collected for t
ransplant in order to establish a minimum threshold for rapid hematopo
ietic recovery. Design and Methods. From January 1995 to November 1996
, a consecutive series of 67 patients, with hematologic and solid tumo
rs underwent autologous PBSC transplantation. Collection of PBSC was p
erformed after mobilization with granulocyte-colony stimulating factor
(G-CSF) or with chemotherapy (CT) plus G-CSF. We calculated the facto
rs that influenced PBSC collection, the kinetics of granulocyte and pl
atelet recovery and the threshold value of CD34(+) cells for a rapid r
ecovery. The data were analyzed by means of multivariate Cox regressio
n model and the receiver operating characteristic (ROC) methodology. R
esults. Our results showed that mobilization with chemotherapy plus G-
CSF was associated with a higher yield of PBSC in comparison with mobi
lization with G-CSF alone. Disease status, fewer cycles of conventiona
l prior chemotherapy and absence of prior radiation therapy also influ
enced the yield of PBSC. The number of CD34(+) cells, CD34(+)CD33(-) c
ell subsets, the mobilization schedule, and the conditioning regimen c
orrelated significantly with time to hematopoietic recovery. In the mu
ltivariate analysis only the CD34(+)CD33(-) cell content and the total
number of CD34(+) were related with rapid neutrophil and platelet rec
overy, respectively. Use of G-CSF after transplant significantly short
ened the neutrophil recovery time only in patients transplanted with s
uboptimal dose of PBSC. Interpretation and Conclusions. These data sug
gest the utility of quantitation of CD34(+) cells subsets to predict q
uick engraftment. (C)1998, Ferrata Storti Foundation.